MICROVESICLE MEMBRANE PROTEIN AND APPLICATION THEREOF
Microvesicles play essential roles in disease progression. The present invention provides a microvesicle membrane protein and application thereof. Disclosed is method comprises phosphorylated CSElL (cellular apoptosis susceptibility protein or CSElL-binding agents for microvesicle isolation, analysis, or binding for disease diagnosis or treatment.In general, the invention discloses novel method comprising antibodies against CSElL or phosphorylated CSElL for microvesicle isolation, analysis, or binding for disease diagnosis or treatment.
Microvesicle proteins especially microvesicle proteins that localized in microvesicle membrane are valuable biomarkers for disease diagnosis. Also, agents that can bind With microvesicle membrane protein can be use for medical imaging or disease treatment.
Another surprising finding in the present invention is that CSElL is linked to ERK signaling pathWay, CSElL is phosphorylated upon ERK activation, phosphorylated CSElL is present in sera from cancer patients, and assay of serum phosphorylated CSElL is superior to assay of serum CSElL for cancer diagnosis.
Another surprising finding in the present invention is that CSElL is linked to Ras-ERK signaling and cancer metastasis. The Ras-ERK pathWay is a potential targets for development of molecularly targeted drug for cancer. CSElL is linked to Ras ERK signaling and cancer metastasis.
In the present invention is that CSElL is localized in microvesicle membrane, and anti CSE1L antibodies can target tumor. The shedding microvesicles are Widespread on the membrane of tumor cells and the shed microvesicles may remain in the extracellular environment around tumor cells; hence, microvesicle membrane proteins may be the potential targets for cancer therapy.